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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which results in chromosome instability, and so may well lead to tumorigenesis. This protein was identified to affiliate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential part in advertising mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are already described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data isn't still obtainable.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally incorporates the epitope recognized with the antibody.
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This guide outlines further more doable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This tutorial outlines even further probable optimization of mobile and immunoassay parameters to be certain the very best final results are...
Antibodies bound to the blocking peptide now not bind to the epitope around the goal protein. By comparing the staining from the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose members are characterized by a extraordinary periodicity in protein abundance in the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show distinct expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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